Role of metallothionein against oxidative stress in the musselMytilus galloprovincialis

• DOI: 10.1152/ajpregu.1999.277.6.r1612
• Publication Date: 2017-12-25T04:52:39Z
• Authors: Aldo Viarengo; Bruno Burlando; Maria Cavaletto; Barbara Marchi; Enrica Ponzano; Julián Blasco
• Keywords: Metallothionein; Malondialdehyde; Detoxification

Metallothionein (MT) is a sulfhydryl-rich protein involved mainly in heavy metal homeostasis and detoxification. In this study, the use of mussel as an experimental model allowed us to test MT antioxidant properties at molecular, cellular, organism level. induction was achieved by exposure Cd (200 μg/l) aquaria for 7 days followed detoxification sea 28 days. Cd-preexposed nonexposed mussels were then treated with Fe (300–600 3 Biochemical assays on digestive gland tissue showed that treatment led significant increase oxyradical production malondialdehyde level only not preexposed Cd. The Cd-dependent resistance oxidative stress ascribed induction, produced no variation reduced glutathione major enzymes. Digital imaging isolated cells lower rise higher viability from after treatments 0.5–5 mM H 2 O . Analyses whole organisms anoxic survival lowered had been Fe, but such effect less pronounced compared nonpreexposed ones. conclusion, data suggest role MT, which seems occur through scavenging able protect both entire stress.