Microarray analysis of the transcriptome of the subfornical organ in the rat: regulation by fluid and food deprivation

• DOI: 10.1152/ajpregu.90560.2008
• Publication Date: 2008-10-01T21:35:33Z
• Authors: Charles Hindmarch; Mark Fry; Song T. Yao; Pauline M. Smith; David Murphy; Alastair V. Ferguson
• Keywords: Male; 0301 basic medicine; 2. Zero hunger; 570; Water Deprivation; Gene Expression Profiling; 610; Nerve Tissue Proteins; Rats; Rats, Sprague-Dawley; 03 medical and health sciences; Gene Expression Regulation; Animals; RNA, Messenger; Food Deprivation; Oligonucleotide Array Sequence Analysis; Subfornical Organ

We have employed microarray technology using Affymetrix 230 2.0 genome chips to initially catalog the transcriptome of subfornical organ (SFO) under control conditions and also evaluate changes (common differential) in gene expression induced by challenges fluid food deprivation. identified a total 17,293 genes tagged as present one our three experimental conditions, transcripts, which were then used basis for further filtering statistical analysis. In total, 46 was changed SFO following dehydration compared with animals (22 upregulated 24 downregulated), largest change being greater than fivefold increase brain-derived neurotrophic factor (BDNF) expression, while significant calcium-sensing (upregulated) apelin (downregulated) receptors reported. contrast, deprivation caused twofold 687 transcripts (222 465 including reductions vasopressin, oxytocin, promelanin concentrating hormone, cocaine amphetamine-related transcript (CART), endothelin type B receptor, well increases GABA(B) receptor. Of these regulated we 37 that are commonly fasting dehydration, nine uniquely 650 fasting. found five differentially BDNF CART. studies first time described rat addition genes, is significantly modified either water or