Effect of pyocyanin and 1-hydroxyphenazine on in vivo tracheal mucus velocity

• DOI: 10.1152/jappl.1989.67.1.316
• Publication Date: 2017-12-21T11:22:20Z
• Authors: N. C. Munro; A. Barker; A. Rutman; G. Taylor; D. Watson; W. J. McDonald-Gi...; R. Towart; W. A. Taylor; R. Wilson; P. J. Cole
• Keywords: Pyocyanin; Respiratory tract; Bolus (digestion); Mucociliary clearance

Products of the bacterium Pseudomonas aeruginosa have been shown to slow beating human respiratory tract cilia in vitro. We tested effects two these compounds, pyocyanin and 1-hydroxyphenazine (given as a bolus dose dissolved 2 microliters Ringer solution), on tracheal mucus velocity radiolabeled erythrocytes anesthetized guinea pigs. 1-Hydroxyphenazine (200 ng) caused rapid slowing (maximum fall 47% at 20 min) with recovery by 1 h. The effect was slower onset, 600 ng causing 60% reduction 3 h, no occurred. A combination produced an initial equivalent same given alone, but later attributed greater than administered alone. This study demonstrates one mechanism which products P. may facilitate its colonization tract.