Cardiopulmonary effects of granulocyte colony-stimulating factor in a canine model of bacterial sepsis

• DOI: 10.1152/jappl.1994.77.5.2366
• Publication Date: 2017-12-22T10:20:03Z
• Authors: P. Q. Eichacker; Y. Waisman; C. Natanson; A. Farese; W. D. Hoffman; S. M. Banks; T. J. MacVittie
• Keywords: Microgram

We investigated the effects of recombinant granulocyte colony-stimulating factor (G-CSF) in a canine model septic shock. Awake 2-yr-old beagles were studied before and after intraperitoneal placement an Escherichia coli-infected clot. Nine days until 3 clot placement, animals received daily high-dose (G-CSF (5 microgram/kg body wt; n = 17), low-dose G-CSF (0.1 or control protein micrograms/kg 20). Survival rate was greater (P < 0.04, Wilcoxon test) group (14/17) than (10/17) (12/20) groups. High-dose improved cardiovascular function, as evidenced by increased left ventricular ejection fraction (day 1 clot; P 0.001) mean arterial pressure 2; 0.02) compared with peripheral neutrophils (-3 days) (2 h to 4 produced more rapid rise 2) fall 4) alveolar neutrophil numbers decreased serum endotoxin (2-8 h; 0.002) tumor necrosis levels lowered blood bacteria counts (2-6 0.04) Thus, this model, sufficient increase during peritonitis shock enhances host defense, reduces cytokine (tumor factor) levels, improves function survival.