Syntaxin 1B is important for mouse postnatal survival and proper synaptic function at the mouse neuromuscular junctions

• DOI: 10.1152/jn.00577.2015
• Publication Date: 2015-07-23T01:50:18Z
• Authors: Yuan-Ju Wu; Rocio Tejero; Marife Arancillo; Gülcin Vardar; Tatiana Korotkova; Michael Kintscher; Dietmar Schmitz; Alexey Ponomarenko; Lucia Tabares; Christian Rosenmund
• Keywords: Forebrain

STX1 is a major neuronal syntaxin protein located at the plasma membrane of tissues. Rodent has two highly similar paralogs, STX1A and STX1B, that are thought to be functionally redundant. Interestingly, some studies have shown distribution patterns STX1B central peripheral nervous systems only partially overlapped, implying there might differential functions between these paralogs. In current study, we generated an knockout (KO) mouse line studied impact removal in neurons several brain regions neuromuscular junction (NMJ). We found either complete or selective it from forebrain excitatory mice caused premature death. Autaptic hippocampal striatal cultures derived KO still maintained efficient neurotransmission compared with wild-type heterozygous mice. examining high-density cerebellar revealed decrease spontaneous GABAergic transmission frequency, which was most likely due lower number cultures, suggesting essential for survival vitro. Moreover, our study also demonstrated although dispensable formation NMJ, required maintain efficiency nerve-muscle synapse.