Identification of Two Types of Inspiratory Pacemaker Neurons in the Isolated Respiratory Neural Network of Mice

• DOI: 10.1152/jn.2001.86.1.104
• Publication Date: 2017-12-21T10:54:56Z
• Authors: Muriel Thoby-Brisson; Jan-Marino Ramirez
• Keywords: CNQX; Bursting; Tetrodotoxin

In the respiratory network of mice, we characterized with whole cell patch-clamp technique pacemaker properties in neurons discharging phase inspiration. The was isolated a transverse brain stem slice containing pre-Bötzinger complex (PBC), presumed site for rhythm generation. After blockade activity 6-cyano-7-nitroquinoxalene-2,3-dione (CNQX), 18 52 inspiratory exhibited endogenous activity, which voltage dependent, could be reset by brief current injections and entrained repetitive stimuli. group ( n = 18), eight generated bursts (0.43 ± 0.03 s) at relatively high frequency (1.05 0.12 Hz) CNQX. These resembled that these intact during interval between two bursts. Cadmium (200 μM) altered but did not eliminate this bursting while 0.5 μM tetrodotoxin suppressed activity. Another set (10 18) CNQX longer (1.57 0.07 lower (0.35 0.01 Hz). population This blocked 50–100 cadmium or tetrodotoxin. We conclude neural contains types properties. activities might have different functions within network.