Molecular organization of adrenergic and noradrenergic neurons in the mouse ventrolateral medulla

• DOI: 10.1152/physiol.2024.39.s1.1195
• Publication Date: 2024-05-21T14:57:50Z
• Authors: Daniel Stornetta; Dana Schwalbe; Ruei-Jen Abraham-Fan; George Souza; Maira Jalil; Maisie Crook; John Campbell
• Keywords: Medulla; Adrenergic Neurons

Catecholaminergic neurons in the ventrolateral medulla (VLM) regulate many important aspects of physiology, including sympathetic control blood pressure, glucose homeostasis, immune function, and neuroendocrine responses to inflammation stress. Previous studies have parsed catecholaminergic VLM into distinct sub-populations based on neurochemical content, connectivity, response activation autonomic reflexes. However, there is no consensus for molecular organization neurons. In this study, we used high-throughput single nuclei RNA sequencing combined with anatomically- genetically-targeted cell-sorting spinally-projecting systematically characterize C1 A1 mouse VLM. Our results, an analysis 894 derived from a dataset 114,805 single-nuclei transcriptomes, indicate that contains 7 molecularly subpopulations neurons, 5 adrenergic subtypes 2 noradrenergic identified by expression enzymes involved production catecholamine ( Th, Dbh) vesicular transporters glutamate Slc6a17) noradrenaline Slc6a2). Neurons projections spinal cord were present 4 cell clusters, 1 cluster. We histologically verified two novel markers Hk2) Eya1) using fluorescent situ hybridization. Hk2 was found primarily Dbh+ rostral VLM, both bulbospinal non-bulbospinal as well majority Pnmt+ C2/C3 regions. Eya1 co-localized Dbh Slc6a2 consistently caudal aligning location whereas signal diffuse Collectively, shows enriched judged conventional criteria irrespective cell's medulla, brainstem provides marker These data show are spatially intermixed gene can be dissect function future studies. This work supported National Institute Health (NIH) R01 HL148004 SBGA; HL153916, Pathway Stop Diabetes Initiator Award 1-18-INI-14, UVA 3Cavaliers grant JNC. full abstract presented at American Physiology Summit 2024 meeting only available HTML format. There additional versions or content abstract. not peer review process.