Divergent multiomic acute exercise responses reveal the impact of sex as a biological variable

• DOI: 10.1152/physiolgenomics.00055.2024
• Publication Date: 2025-02-27T15:30:38Z
• Authors: Kaleen M. Lavin; Samia M. O’Bryan; Khyatiben V. Pathak; Krystine Garcia-M...; Zachary A. Graham; Jeremy S. McAdam; Devin J. Drummer; Margaret B. Bell; Christian J. Kelley; Manoel E. Lixandrão; Brandon Peoples; Regina S. Seay; Anakaren R. Torres; Rebecca Reiman; Eric Alsop; Elizabeth Hutchins; Anna Bonfitto; Jerry Antone; Joanna Palade; Kendall Van Keure...; Matthew J. Huente...; Patrick Pirrotte; Timothy Broderick; Marcas M. Bamman
• Keywords: Exercise physiology; Sex characteristics; Physical exercise

The majority of exercise physiology research has been conducted in males, resulting a skewed biological representation how impacts the physiological system. Extrapolating male-centric findings to females is not universally appropriate and may even be detrimental. Thus, addressing this imbalance taking into consideration sex as variable mandatory for optimization precision interventions and/or regimens. Our present analysis focused on establishing multiomic profiles young, exercise-naïve males (n=23) (n=17) at rest following acute exercise. Sex differences were characterized baseline using skeletal muscle extracellular vesicle transcriptomics, whole blood methylomics, serum metabolomics. Sex-by-time response revealed notable overlap, well divergent molecular responses between females. An exploratory comparison two combined regimens (high-intensity: HITT traditional: TRAD) was then performed singular value decomposition, revealing latent data structures that suggest complex dose-by-sex interaction These lay groundwork an understanding key exposure sexes. This leveraged designing optimal training strategies, common interplay guiding responses, elucidating role hormones other sex-specific attributes chronic