Detection of Urinary Metabolomics before and after Pringle Maneuver-Induced Liver Ischemia and Reperfusion Injury in Rats Using Gas Chromatography-Mass Spectrometry

• DOI: 10.1155/2013/792391
• Publication Date: 2013-09-23T21:19:19Z
• Authors: Liyan Chen; Zhenchao Luo; Wenguang Fu; Xinxin Liao; Zhonglin Cui; Jie Zhou
• Keywords: Male; 0301 basic medicine; Alanine; Alanine Transaminase; Bilirubin; Gas Chromatography-Mass Spectrometry; Rats; 3. Good health; Rats, Sprague-Dawley; 03 medical and health sciences; Liver; Glycerophosphates; Reperfusion Injury; Pyruvic Acid; Metabolome; Serine; Animals; Metabolomics; Lactic Acid; Research Article

Background. Metabolomics studies can quantitatively detect the dynamic metabolic response of living systems.Objective. To detect urinary metabolomics after hepatic ischemia/reperfusion (I/R) injury induced by the Pringle maneuver using gas chromatography-mass spectrometry (GC-MS).Methods. Male Sprague-Dawley rats () were randomly divided into 4 groups (/group): sham operation, day 1, day 3, and day 5. Rats in the day 1, day 3, and day 5 groups underwent the Pringle maneuver. Serum alanine transaminase (ALT) and total bilirubin (TBIL) were measured, and hematoxylin and eosin (HE) staining of the liver tissue was performed. GC-MS was used to detect urinary metabolomics.Results. Compared with the sham group, the serum ALT and TBIL levels at day 1 were significantly elevated () and then decreased and reached close to normal levels at day 5. GC-MS detected 7 metabolites which had similar changes as those of liver tissue revealed by histological examination. Significant differences in lactic acid, pyruvic acid, alanine, serine, and glycerol-3-phosphate were found among the groups (). Principle component analysis showed that 7 metabolites distinguished the day 1 and day 3 groups from the sham group.Conclusions. Noninvasive urinary metabolomic analysis is a potential means for the early detection and diagnosis of hepatic I/R injury.