Protection from Endotoxic Uveitis by Intravitreal Resolvin D1: Involvement of Lymphocytes, miRNAs, Ubiquitin‐Proteasome, and M1/M2 Macrophages

• DOI: 10.1155/2015/149381
• Publication Date: 2015-01-15T21:00:41Z
• Authors: S. Rossi; C. Di Filippo; C. Gesualdo; N. Potenza; A. Russo; M. C. Trotta; M. V. Zippo; R. Maisto; F. Ferraraccio; F. Simonelli; M. D’Amico
• Keywords: Proinflammatory cytokine; Macrophage inflammatory protein

This study investigated the protective effects of intravitreal Resolvin D1 (RvD1) against LPS-induced rat endotoxic uveitis (EIU). RvD1 was administered into right eye at a single injection 5 μL volume containing 10-100-1000 ng/kg 1 h post-LPS (200 μg, Salmonella minnesota) thefootpad Sprague-Dawley rats. 24 later, enucleated and examined for clinical, biochemical, immunohistochemical evaluations. significantly dose-dependently decreased clinical score attributed to EIU, starting from dose 10 further by 100 1000 ng/kg. These were accompanied changes in four important determinants immune-inflammatory response within eye: (i) B T lymphocytes, (ii) miRNAs pattern, (iii) ubiquitin-proteasome system (UPS), (iv) M1/M2 macrophage phenotype. LPS+RvD1 treated rats showed reduced presence lymphocytes upregulation miR-200c-3p, miR 203a-3p, 29b-3p, 21-5p compared LPS alone. paralleled decreases ubiquitin, 20S 26S proteasome subunits, M1, increased M2 ocular tissues. Accordingly, levels cytokine TNF-α, chemokines MIP1-α NF-κB reduced.