Decolorization of Anthraquinonic Dyes from Textile Effluent Using Horseradish Peroxidase: Optimization and Kinetic Study

• DOI: 10.1155/2015/371625
• Publication Date: 2015-01-19T21:00:36Z
• Authors: Nataša Ž. Šekuljica; Nevena Ž. Prlainović; Andrea B. Stefanović; Milena G. Žuža; Dragana Z. Čičkarić; Dušan Ž. Mijin; Zorica D. Kneževi...
• Keywords: Horseradish peroxidase; Acid dye

Two anthraquinonic dyes, C.I. Acid Blue 225 and Violet 109, were used as models to explore the feasibility of using horseradish peroxidase enzyme (HRP) in practical decolorization dyes wastewater. The influence process parameters such concentration, hydrogen peroxide temperature, dye pH was examined. temperature activity profiles similar for both dyes. Under optimal conditions, 94.7% 109 from aqueous solution decolorized (treatment time 15 min, concentration 0.15 IU/mL, 0.4 mM, 30 mg/L, 4, 24°C) 89.36% (32 0.04 5, 24°C). mechanism reactions has been proven follow two substrate ping-pong with inhibition, revealing formation a nonproductive or dead-end complex between HRP H 2 O oxidized form enzyme. Both chemical oxygen demand total organic carbon values showed that there reduction toxicity after enzymatic treatment. This study verifies viability use wastewaters treatment