CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells

• DOI: 10.1155/2016/1542786
• Publication Date: 2016-09-25T17:00:34Z
• Authors: Xing Lin; Qianshun Chen; Chen Huang; Xunyu Xu
• Keywords: MTT assay

Lung cancer is one of the most common cancers in world. Cylindromatosis (CYLD) a deubiquitination enzyme and contributes to degradation ubiquitin chains on RIP1. The aim present study investigate levels CYLD lung patients explore molecular mechanism pathogenesis. were detected human tissues paired paracarcinoma by real-time PCR western blotting analysis. proliferation cells was determined MTT assay. Cell apoptosis necrosis FACS results demonstrated that low clinical carcinoma specimens. Three pairs siRNA used knock down endogenous cells. Knockdown promoted cell Otherwise overexpression induced TNF- α -induced death A549 H460 Moreover, CYLD-overexpressed treated with 10 μ M z-VAD-fmk for 12 hours result revealed significantly enhanced. Additionally, mediated receptor-interacting protein 1 (RIP-1) kinase. Our findings suggested potential target therapy cancers.