Data from Transcription Factor NF-Y Induces Apoptosis in Cells Expressing Wild-Type p53 through E2F1 Upregulation and p53 Activation

• DOI: 10.1158/0008-5472.c.6501461.v1
• Publication Date: 2024-03-12T19:19:43Z
• Authors: Aymone Gurtner; Paola Fuschi; Fabio Martelli; Isabella Manni; Simona Artuso; Giacoma Simonte; Valeria Ambrosino; Annalisa Antonini; Valentina Folgiero; Rita Falcioni; Ada Sacchi; Giulia Piaggio
• Keywords: E2F1

<div>Abstract<p>The CCAAT-binding transcription factor NF-Y plays a central role in regulating cellular proliferation by controlling the expression of genes required for cell-cycle progression such as <i>cyclin A</i>, B1</i>, B2</i>, <i>cdc25A</i>, <i>cdc25C</i>, and <i>cdk1</i>. Here we show that unrestricted activity leads to apoptosis an E2F1- wild-type p53 (wtp53)-dependent manner. Unrestricted induced increase E2F1 mRNA protein levels. Furthermore, directly bound promoter this correlated with appearance open chromatin marks. The ability induce was impaired cells lacking wtp53. Moreover, overexpression elicited phosphorylation wt p53Ser18 E2F1-dependent Our findings establish acts upstream p53-mediated apoptosis. <i>Cancer Res; 70(23); 9711–20. ©2010 AACR</i>.</p></div>