FIGURE 2 from A Druggable FOXA1-Glucocorticoid Receptor Transcriptional Axis Drives Tumor Growth in a Subset of Non-Small Cell Lung Cancer

• DOI: 10.1158/2767-9764.24101072.v1
• Publication Date: 2023-09-07T14:21:37Z
• Authors: M. Dorso; Payal T. Patel; Aleksandr Pankov; Jacob A. Boyer; Rajesh K. Soni; Isabella S. Del P...; Omar Hayatt; Amanda Kulick; Connor J. Hagen; Elisa de Stanchina; Melissa R. Junttila; Anneleen Daemen; Lori S. Friedman; Ronald C. Hendric...; Sarat Chandarlapaty
• Keywords: FOXA1

<p>Identification of a FOXA1–GR interaction through endogenous purification FOXA1-associated proteins using RIME. <b>A,</b> Schematic overview the RIME assay. <b>B,</b> Graphic representation GR protein sequence coverage unique peptides identified by LC/MS-MS in H441, HCC-44, and H3255 FOXA1 samples. Yellow regions string represent alignment peptide sequences to Mascot database for protein. Full list found <a href="#SMT2" target="_blank">Supplementary Table S2</a>. <b>C,</b> as B) H441 <b>D,</b> Co-IP FOXA1-dependent NSCLC cell lines. was immunoprecipitated full serum-containing media GR, FOXA1, GAPDH were immunoblotted whole-cell lysate (input), IgG IP, IP fractions. <b>E,</b> Volcano plot displaying significantly upregulated (red) or downregulated (green) genes H2009 doxycycline-inducible shFOXA1 cells compared with shRenilla control following 72 hours 1 μg/mL doxycycline treatment. <b>F,</b> Gene ontology pathway analysis significant differentially regulated between cells. <b>G,</b> qPCR fold change <i>FOXA1</i>, <i>SGK1,</i> <i>DUSP1</i> mRNA levels normalized treated 48 hours. Values correspond mean three replicates ± SEM. <i>P</i> value calculated Student two-tailed <i>t</i> test (<i>P</i> < 0.01).</p>