FIGURE 3 from Nanoparticle Delivery of Immunostimulatory Alu RNA for Cancer Immunotherapy

• DOI: 10.1158/2767-9764.24107113.v1
• Publication Date: 2023-09-08T14:20:23Z
• Authors: Kyle M. Garland; Alexander J. Kwia...; John T. Tossberg; Philip S. Crooke; Thomas M. Aune; John T. Wilson

<p><i>In vitro</i> characterization of AluJb RNA (Left Arm)/D-PDB. <b>A,</b> Agarose gel of AluJb RNA (Left Arm) with and without various amounts of D-PDB, as indicated. 1 µg RNA/lane. The TrackIt 100 bp DNA Ladder was employed. <b>B,</b> DLS analysis of Alu-NPs and Edited Alu-NPs (i.e., AluJb RNA (Left Arm)/D-PDB at an N/P ratio of 4 and Inactive AluJB (Left Arm)/D-PDB at an N/P ratio of 4) relative to D-PDB. <b>C,</b> TEM images of D-PDB NPs, Edited Alu-NPs, and Alu-NPs. Scale bars, 100 µm. RAW-Dual (<b>D</b>) and THP1-Dual (<b>E</b>) reporter cell assays of Alu-NPs, Edited Alu-NPs, D-PDB, D-B Alu NPs, and Free AluJB RNA (Left Arm). All curves are normalized to the maximum value of the Alu-NPs. All experiments were performed at least twice.</p>