FIGURE 5 from Lung Fibroblasts Take up Breast Cancer Cell-derived Extracellular Vesicles Partially Through MEK2-dependent Macropinocytosis

• DOI: 10.1158/2767-9764.25041203.v1
• Publication Date: 2024-01-22T14:20:18Z
• Authors: Yuhao Wan; Yue Zhao; Minghui Cao; Jingyi Wang; Sheila V. Tran; Zhixuan Song; Brent W. Hsueh; Shizhen Emily Wang
• Keywords: Pinocytosis

<p>Trametinib interferes with macropinocytosis in lung fibroblasts while Copanlisib both and clathrin-mediated endocytosis. <b>A,</b> Quantification of the cellular uptake indicated markers or without Trametinib treatment. Data are presented as mean ± SD (<i>n</i> = 3 wells per group; 5 images well). Faint symbols represent all 15 group. Dark value from each well. **, <i>P</i> < 0.01; ns, not significant. <b>B,</b> Representative markers. Images were captured at 10 ×. Green, FITC-transferrin, Alexa488-BSA FITC-dextran; blue, Hoechst 33342. Scale bar, 100 µm.</p>