FIGURE 2 from Lung Fibroblasts Take up Breast Cancer Cell-derived Extracellular Vesicles Partially Through MEK2-dependent Macropinocytosis

• DOI: 10.1158/2767-9764.25041212.v1
• Publication Date: 2024-01-22T14:20:24Z
• Authors: Yuhao Wan; Yue Zhao; Minghui Cao; Jingyi Wang; Sheila V. Tran; Zhixuan Song; Brent W. Hsueh; Shizhen Emily Wang
• Keywords: Pinocytosis

<p>Human lung fibroblasts take up MDA-MB-231 EVs via dynamin- and caveolae-dependent endocytosis macropinocytosis. <b>A,</b> Fibroblasts were pretreated with 80 µmol/L Dynasore or an equal volume of DMSO (as a control) for 24 hours then incubated 10 µg/mL 6 in the continuous presence control (<i>n</i> = 3 wells per group; 5 images well). Data are presented as mean ± SD. ***, <i>P</i> < 0.001. <b>B,</b> Cells 50 EIPA, CPZ, 200 Genistein drug 0.001; ns, not significant. <b>C,</b> Representative Dynasore-, EIPA-, CPZ-, Genistein-treated cells untreated cells. Images captured at ×. Green, CFSE; yellow, DiI; blue, Hoechst 33342. Scale bar, 100 µm.</p>