FIGURE 7 from Overcoming Xenoantigen Immunity to Enable Cellular Tracking and Gene Regulation with Immune-competent “NoGlow” Mice

• DOI: 10.1158/2767-9764.25569254.v1
• Publication Date: 2024-04-09T15:21:44Z
• Authors: Timothy N. Trotter; Andrea Wilson; Jason McBane; Carina E. Dagotto; Xiao-Yi Yang; Jun-Ping Wei; Gangjun Lei; Hannah Thrash; Joshua C. Snyder; Herbert Kim Lyerly; Zachary C. Hartman
• Keywords: Tracking (education)

<p>Summary of tumor behavior in differentially tolerant animal models. <b>A,</b> Xenoantigen-bearing cells implanted orthotopically into immune-deficient mice typically grow and metastasize as expected can be easily observed with fluorescence/bioluminescence. <b>B,</b> Conversely, immune-competent animals the presence foreign immunogens often results complete rejection primary tumor, or severely restricts expression immunogenic antigens limits utility reporter proteins. <b>C,</b> However, NoGlow are to GFP, Luciferase, rtTA, thus allowing growth <i>de novo</i> metastasis xenoantigen-expressing cells.</p>