FIGURE 4 from Identification of MUC1-C as a Target for Suppressing Progression of Head and Neck Squamous Cell Carcinomas

• DOI: 10.1158/2767-9764.25817986.v1
• Publication Date: 2024-05-14T14:20:32Z
• Authors: Ayako Nakashoji; Naoki Haratake; Atrayee Bhattacharya; Weipu Mao; Kangjie Xu; Keyi Wang; Tatsuaki Daimon; Hiroki Ozawa; Keisuke Shigeta; Atsushi Fushimi; Nami Yamashita; Yoshihiro Morimoto; Mototsugu Shimokawa; Shin Saito; Ann Marie Egloff; Ravindra Uppaluri; Mark D. Long; Donald Kufe

<p>MUC1-C/STAT1 signaling regulates ∆Np63 expression. <b>A,</b> CAL27/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for <i>∆Np63</i> gene transcription (left) and mRNA (right) levels. The results (mean ± SD of four determinations) are expressed as relative levels compared with that obtained for vehicle-treated cells (assigned a value of 1). <b>B,</b> CAL27/CshRNA and CAL27/STAT1shRNA cells were analyzed for <i>∆Np63</i> gene transcription (left) and mRNA (right) levels. The results (mean ± SD of four determinations) are expressed as relative levels compared with that obtained for vehicle-treated cells (assigned a value of 1). <b>C,</b> Schema of the <i>∆Np63</i> gene with highlighting localization of the PLS region that contains potential STAT1 binding motifs. <b>D,</b> Soluble chromatin from CAL27/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days was precipitated with anti-MUC1-C and anti-STAT1. The DNA samples were amplified by qPCR with primers for the <i>∆Np63</i> PLS region. The results (mean ± SD of three determinations) are expressed as percentage of the input DNA for each sample. <b>E,</b> CAL27 cells expressing the indicated vectors were treated with vehicle or DOX for 7 days. Lysates were immunoblotted with antibodies against the indicated proteins. <b>F,</b> Lysates from CAL27/CshRNA and CAL27/STAT1shRNA cells were immunoblotted with antibodies against the indicated proteins.</p>