Effect of nitroxyl on human platelets function

• DOI: 10.1160/th05-01-0062
• Publication Date: 2005-08-11T10:27:27Z
• Authors: Emilse Bermejo; Fabiana Alberto; Ruth E. Rosenstein; Sara E. Bari; María A. Lazzari; Daniel A. Sáenz
• Keywords: Sodium nitroprusside; Nitroxyl; CD63

Summary There is a growing body of evidence on the role nitric oxide (NO) in human platelet physiology regulation. Recently, interest has developed functional an alternative redox form NO, namely nitroxyl (HNO/NO-), because it formed by number diverse biochemical reactions. The aim present study was to comparatively analyze effect HNO and NO several parameters platelets. For this purpose, sodium trioxodinitrate (Angeli’s salt, AS) nitroprusside (SNP) were used as releasers, respectively. Both AS SNP significantly inhibited aggregation ATP release induced different agonists adrenaline. or did not modify expression glycoproteins (Ib, IIb-IIIa, Ia-IIa, IV), whereas they substantially decreased levels CD62P, CD63 PAC-1 (a activated glycoprotein IIb/IIIa epitope) after stimulation with ADP. increased cGMP accumulation 1H-[1,2,4]oxadiazolo [4,3-a] quinoxalin-1-one (ODQ)-sensitive manner. However, while L-cysteine reduced AS, parameter. Accordingly, differential observed antiaggregatory both compounds. In summary, these results indicate that effective inhibitor aggregation.