Different expression of proteins in platelets from aspirin-resistant and aspirin-sensitive patients

• DOI: 10.1160/th09-05-0290
• Publication Date: 2009-11-17T07:04:46Z
• Authors: Carlos Macaya; Luis Azcona; Javier Modrego; Estela Mahillo; Esther Bernardo; Antonio Fernández...; Antonio López-Farré; Petra Mateos-Cáceres
• Keywords: Gelsolin; P-selectin

The aim of the present study was to analyse differences in protein expression profile between platelets from aspirin (ASA)-resistant patients and ASA-sensitive patients. We analysed 51 clinically stable coronary ischaemic taking ASA (100 mg/day) divided into ASA-resistant (n=25) (n=26) based on a platelet functionality test (PFA-100). Proteins associated with cytoskeleton, energetic metabolism, oxidative stress, inflammation cell survival were by two-dimensional electrophoresis mass spectrometry. two gelsolin precursor isotypes one F-acting capping isotype decreased (p<0.05). glyceraldehyde 3-phosphate dehydrogenase increased (1751.1 + or - 220.6 vs. 4273.3 971.7, 95% confidence interval [CI] 1815.11 4061.2, p=0.001). It accompanied reduced activity 1,6-bisphosphate aldolase without changes content pyruvate. A gluthathione-S-transferase disulfide isomerase 1 found platelets. chloride intracellular channel (21.3 3.8 48.8 6.0, CI 29.5 45.95, p=0.03) while HSP60 HSP71 decreased. No observed proteins inflammation. In conclusion, are different terms level mechanisms such as stress which may be their ability respond ASA.