Resistin Promotes Endothelial Cell Activation

• DOI: 10.1161/01.cir.0000084503.91330.49
• Publication Date: 2003-07-22T00:34:50Z
• Authors: Subodh Verma; Shu-Hong Li; Chao-Hung Wang; Paul W.M. Fedak; Ren-Ke Li; Richard D. Weisel; Donald A.G. Mickle
• Keywords: Resistin

Adipocyte-derived hormones may represent a mechanism linking insulin resistance to cardiovascular disease. In the present study, we evaluated direct effects of resistin, novel adipocyte-derived hormone, on endothelial activation.Endothelial cells (ECs) were incubated with human recombinant resistin (10 100 ng/ML, 24 hours), and endothelin-1 (ET-1) release, ET-1 mRNA expression, nitric oxide (NO) production assessed. Transient transfection assays used evaluate transcription gene promoter. Furthermore, AP-1-mutated promoter evaluated. The expression vascular cell adhesion molecule (VCAM-1) monocyte chemoattractant chemokine (MCP-1) studied in addition CD40 receptor, ligand-induced MCP-1 tumor necrosis factor receptor-associated factor-3 (TRAF3), an inhibitor signaling. Incubation ECs resulted increase release no change NO production. Whereas treatment activity, was inactive after stimulation. Additionally, resistin-treated showed increased VCAM-1 MCP-1, concomitant reductions TRAF-3 expression. Resistin did not alter receptor expression; however, ligand induced production.The adipokine exerts promote EC activation by promoting part inducing activity via AP-1 site. upregulates molecules chemokines downregulates TRAF-3, this fashion, be mechanistically linked disease metabolic syndrome.