Therapeutic Potential of Ex Vivo Expanded Endothelial Progenitor Cells for Myocardial Ischemia

• DOI: 10.1161/01.cir.103.5.634
• Publication Date: 2012-06-12T00:09:32Z
• Authors: Atsuhiko Kawamoto; Heon-Cheol Gwon; Hideki Iwaguro; Jun-Ichi Yamaguchi; Shigeki Uchida; Haruchika Masuda; Marcy Silver; Hong Ma; Marianne Kearney; Jeffrey M. Isner; Takayuki Asahara
• Keywords: Ex vivo

Background —We investigated the therapeutic potential of ex vivo expanded endothelial progenitor cells (EPCs) for myocardial neovascularization. Methods and Results —Peripheral blood mononuclear obtained from healthy human adults were cultured in EPC medium harvested 7 days later. Myocardial ischemia was induced by ligating left anterior descending coronary artery male Hsd:RH-rnu (athymic nude) rats. A total 10 6 EPCs labeled with 1,1′-dioctadecyl-1 to 3,3,3′,3′-tetramethylindocarbocyanine perchlorate injected intravenously 3 hours after induction ischemia. Seven later, fluorescence-conjugated Bandeiraea simplicifolia lectin I administered intravenously, rats immediately killed. Fluorescence microscopy revealed that transplanted accumulated ischemic area incorporated into foci To determine impact on ventricular function, 5 (EPC group) ischemia; other (control received culture media. Echocardiography, performed just before 28 ischemia, disclosed dimensions significantly smaller fractional shortening greater group than control day 28. Regional wall motion better preserved group. After euthanization 28, necropsy examination capillary density Moreover, extent scarring less receiving controls. Immunohistochemistry capillaries positive human-specific cells. Conclusions —Ex incorporate neovascularization have a favorable preservation function.