Biochemical basis for the difference between normal and atherosclerotic arterial fluorescence.

• DOI: 10.1161/01.cir.80.6.1893
• Publication Date: 2011-06-17T20:29:13Z
• Authors: L I Laifer; K M O'Brien; M L Stetz; G R Gindi; T J Garrand; L I Deckelbaum
• Keywords: Arteriosclerosis; Lasers; Aortic Diseases; Lipid Metabolism; 01 natural sciences; Elastin; Microscopy, Fluorescence; 0103 physical sciences; Humans; Collagen; Algorithms; Angioplasty, Balloon; Aorta

The observation that laser-induced fluorescence (LIF) spectra of atherosclerotic and normal artery are different has been proposed as the basis for guiding a "smart" laser angioplasty system. purpose this study was to investigate causes difference in LIF. Helium-cadmium (325 nm) recorded from pure samples known constituents including collagen, elastin, calcium, cholesterol, glycosaminoglycans. Similarities between LIF plaque collagen aorta elastin were noted. spectroscopy then performed on specimens aortic (n = 9) 13) their extracted lipid, elastin. Lipid extraction did not significantly alter or LIF, suggesting minor contribution lipid arterial wall similar whereas collagen. These observations consistent with reported relative collagen-to-elastin content ratio 0.5 7.3 plaque. A classification algorithm developed discriminate based an spectral decomposition. discriminant score formed by (E-C) coefficients used classify 182 spectra. mean E-C value +0.83 +/- 0.04 -0.48 0.07 (p less than 0.001). Classification accuracy 92%.(ABSTRACT TRUNCATED AT 250 WORDS)