Regulation of Fibrillar Collagen Gene Expression and Protein Accumulation in Volume-Overloaded Cardiac Hypertrophy

• DOI: 10.1161/01.cir.95.10.2448
• Publication Date: 2012-06-12T00:41:02Z
• Authors: Takashi Namba; Hiroyuki Tsutsui; Hirofumi Tagawa; Masaru Takahashi; Keiko Saito; Toshiyuki Kozai; Makoto Usui; Kyoko Imanaka-Yos...; Tsutomu Imaizumi; Akira Takeshita
• Keywords: Sirius Red; Pressure overload; Volume overload; Ventricular remodeling

Background Interstitial collagen accumulation has been extensively demonstrated to be increased at both mRNA and protein levels in pressure-overloaded cardiac hypertrophy. However, few data are available regarding the effects of volume overload on myocardial collagens. Methods Results To determine whether alterations collagens may occur volume-overloaded hypertrophy, we measured types I III left ventricular (LV) myocardium rats 3, 7, 28 days after creation an aortocaval (AC) shunt. Eccentric LV hypertrophy was produced with AC shunting. Northern blot analysis RNA extracted from tissue indicated that steady state for type were persistently upregulated shunt compared sham-operated control rats. In contrast, biochemical concentration morphometric fraction comparable between any study time point. Furthermore, immunohistochemical staining Sirius red sections revealed no significant distribution or density fibrillar Tissue collagenase activity not different days. Conclusions Cardiac increases as does pressure overload. contrast upregulation transcriptional result subsequent fibrosis due Therefore, gene expression might