Control of glial cell volume in anoxia. In vitro studies on ischemic cell swelling.

• DOI: 10.1161/01.str.18.3.623
• Publication Date: 2011-06-17T20:08:03Z
• Authors: O Kempski; M Zimmer; A Neu; F von Rosen; M Jansen; A Baethmann
• Keywords: 0301 basic medicine; Kinetics; Osmosis; 03 medical and health sciences; Statistics as Topic; Hypoxia; Neuroglia; Cell Line

Volume regulation of C6 glial cells was studied in anoxia vitro to improve the understanding ischemic cell swelling brain. Contrary vivo conditions, or plus iodoacetate for additional inhibition anaerobic energy metabolism did not induce swelling. However, intracellular K+ markedly decreased while Na+ increased. Induction failure by found prevent regulatory volume decrease on hyposmotic exposure cells, which is regularly observed normoxic control conditions. Hyposmotic led only an initial tendency normalization followed secondary This associated with a net increase that may explain under these circumstances. Maintenance normal size during complete deprivation isotonic medium strongly indicates per se does suffice Cell cerebral ischemia thus likely require mechanisms, most membrane permeability Na+, be caused release and accumulation excitotoxins such as glutamate extracellular K+. Such mechanism would hardly influence homeostasis due large medium-to-cell ratio. The findings demonstrate, nonetheless, significance competent support regulation. concluded from hypotonically suspended iodoacetate.(ABSTRACT TRUNCATED AT 250 WORDS)