Abstract 324: Molecular Imaging of High Risk Atherosclerotic Plaque Using Fibrin-Binding PET Probe

• DOI: 10.1161/atvb.38.suppl_1.324
• Publication Date: 2019-03-12T14:49:15Z
• Authors: David Izquierdo-G...; Himashinie Diyaba...; Ian Ramsay; Nicholas J Rotile; Adam Mauskapf; Ji-Kyung Choi; Thomas Witzel; Valerie Humblet; Anna-Liisa Brownell; Ciprian Catana; Peter Caravan; Ilknur Ay
• Keywords: Abdominal aorta; Ex vivo; Balloon catheter

Objectives: There is a need for better stratification of atherosclerotic patients to identify individuals at high risk cardiovascular event. Two key hallmarks plaque rupture are intraplaque hemorrhage and evidence prior rupture. The protein fibrin strongly associated with both but it not present in earlier stages development, suggesting that imaging would be sensitive versus stable/early disease. Here, we describe properties fibrin-specific PET probe rabbits. Methods: Rabbits were fed cholesterol diet (HCD) 8 weeks followed by 2 normal diet. after the initiation HCD, endothelial denudation abdominal aorta was performed using balloon catheter. At 10 weeks, rabbits randomly divided into (n=8; triggered Russell’s viper venom histamine) control group (n=4), injected fibrin-binding [ 68 Ga]CM-246, scanned on PET/MRI. Then, vessels interest removed ex-vivo PET, autoradiography, histology. Results: had >2-fold increase Ga]CM-246 signal compared (A). 105-120 min post injection, there clear uptake rabbit (B) (C). MR images used differentiate (green arrows) inferior vena cava (blue arrows). T2-dark blood confirmed plaques (D) (E). ToF visualize aortic lumen (F) (G). Ex vivo (H) autoradiography results. Conclusions: We demonstrated detects model can useful tool diagnosis high-risk patients.