Abstract 3045: Dietary Whole-grain Rice Differentially Regulates HDL-associated MicroRNAs And Hepatic Lipase In Healthy Japanese Volunteers.

• DOI: 10.1161/atvb.44.suppl_1.3045
• Publication Date: 2024-07-24T13:03:55Z
• Authors: Maki Tsujita; Kosuke Nakasuka; Clark Massick; Yutaka Miura; Takashi Miida; Kasey C Vickers
• Keywords: Hepatic lipase

Background: The Japanese population consumes a diet high in rice. Dietary whole-grain (brown) rice has advantageous health benefits over white rice, largely predicted to be conferred by brown bran, germ, and fiber. We have previously reported that high-density lipoproteins (HDL) transport microRNAs (miRNA) deliver functional RNA recipient hepatocytes. Here, we hypothesize dietary reorganizes small (sRNA) cargo on circulating HDL regulates systemic lipid metabolism through hepatic HDL-miRNA uptake. Methods: Healthy subjects (n=13, 20-65 y.o., 38.5% male) consumed 150 g/serving for all 3 meals of (1) (2) (3) days with intervals 4 7 days. At day 4, plasma HDL-cholesterol (HDL-C) total lipids were measured direct enzymatic methods. Total was isolated purified size-exclusion chromatography from human plasma. HDL-sRNA sequencing completed using NovaSeq6000 (Illumina). HTGL protein levels ELISA (IBL). Results: Plasma cholesterol, HDL-C, non-HDL-C not immediately affected rice; however, HDL-C increased after delay 10 Nevertheless, HDL-associated miR-203-3p miR-205-5p found significantly decreased consumption compared is target suppress lipase ( LIPC , HTGL). altered but when the switched back returned particles. Conclusions: reduces differentially level putative gene. These results support HDL-miR-203-3p functionality may responsive diets post-transcriptionally regulate gene expression observations can potentially used design supplements function liver control metabolism.