Human Neutrophil Peptides Mediate Endothelial-Monocyte Interaction, Foam Cell Formation, and Platelet Activation

• DOI: 10.1161/atvbaha.111.227116
• Publication Date: 2011-08-05T04:23:23Z
• Authors: Kieran L. Quinn; Melanie Henriques; Arata Tabuchi; Bing Han; Hong Yang; Wei-Erh Cheng; Soumitra Tole; Hanpo Yu; Alice Luo; Emmanuel Charbonney; Elizabeth Tullis; Alan Lazarus; Lisa A. Robinson; Heyu Ni; Blake R. Peterson; Wolfgang M. Kuebler; Arthur S. Slutsky; Haibo Zhang
• Keywords: Monocyte; Foam cell

Neutrophils are involved in the inflammatory responses during atherosclerosis. Human neutrophil peptides (HNPs) released from activated neutrophils exert immune modulating properties. We hypothesized that HNPs play an important role neutrophil-mediated cardiovascular atherosclerosis.We examined of endothelial-leukocyte interaction, platelet activation, and foam cell formation vitro vivo. demonstrated stimulation human coronary artery endothelial cells with clinically relevant concentrations resulted monocyte adhesion transmigration; induction oxidative stress macrophages, which accelerates formation; activation aggregation platelets. The administration superoxide dismutase or anti-CD36 antibody reduced cholesterol efflux. Mice deficient double genes low-density lipoprotein receptor receptor-related protein (LRP), mice a single gene LRP8, only LRP phenotype expressed platelets, showed leukocyte rolling decreased thrombus response to HNP stimulation.HNPs proatherosclerotic properties appear be mediated through LRP8 signaling pathways, suggesting for development diseases.