Abstract 8443: Circulating MicroRNAs Expression in Human Plasma is Dysregulated by Heart Failure

• DOI: 10.1161/circ.124.suppl_21.a8443
• Publication Date: 2024-04-23T09:55:19Z
• Authors: Yuri D alessandra; Antonio P Beltrami; Maria Cristina Ca...; Paolo Devanna; Cinzia Fortini; Fabrizio Cecaro; Roberto Ferrari; Daniela Cesselli; Barbara Toffoletto; Natascha Bergamin; Giulio Pompilio; Maurizio C Capogr...
• Keywords: Human heart

Purpose: Circulating microRNAs (miRNAs) have recently been identified and proposed as novel biomarkers of several diseases. Here we examined miRNAs expression profile heart failure (HF) patients, NYHA class I-IV, in comparison to matched controls. Methods: We analyzed the 365 plasma samples from healthy donors (n= 5) I-IV HF patients 5/group). MiRNAs was evaluated using TaqMan microRNA Arrays followed by a validation step. Results were normalized miR-532-5p an internal experimentally validated calibrator with low dispersion levels null association HF. Validated subjects (n=10) (n=10-14/ group). RESULTS: Of ∼190 expressed able identify 17 dysregulated (14 up- 3 down-regulated), 7 showing no control (miR-154,-299, -376a, -379, -409-5p, -548a -654). Expression analysis 9 these throughout 4 classes showed that miR-154, -221, -409-5p -379 are strongly overexpressed (20 170 folds vs controls) all while miR-124a, -375 -451 exhibit significant downregulation I (40%), IV (50%) III (70 90%) respectively. In agreement prior study, miR- 423-5p exhibited increase subjects, statistically II (3 folds). Interestingly miR-379 marked differences between III. Further, 8 (miR-1, -21, 146a, -548a, -299-5p, -331-5p, -654-5p -376c) differentiated controls (n=14) exhibiting up-regulation 6 40 folds. Conclusions: A miRNA signature consisting enhanced 14 decrease found (NYHA IV). These findings pave way for identification which can be viewed promising failure.