Abstract 9617: Real Angiogenic Potential of Early or Late Outgrowth Endothelial Progenitor Cells is Rigorously Dependent on the Time of Emergence

• DOI: 10.1161/circ.126.suppl_21.a9617
• Publication Date: 2024-04-23T10:06:13Z
• Authors: Yoshiyasu Minami; Makoto Sahara; Toshiaki Nakajima; Masayasu Ikutomi; Toshihiro Morita; Hiroshi Yamashita; Yasunobu Hirata; Ryozo Nagai; Masataka Sata
• Keywords: Progenitor; Endothelial progenitor cell

Background: Recent studies have suggested that late outgrowth endothelial progenitor cells (EPCs) emerging from cultured peripheral blood mononuclear (PBMNCs) might higher angiogenic potential than of classically defined early EPCs (EOCs). However, it still remains undetermined which EPC subpopulations rigorously by the time emergence has highest therapeutic potency. Methods and Results: Human PBMNCs freshly isolated were under cell conditions. EOCs as attached on culture plates at days 3 to 7, forming clusters with cobble stone appearance 10 30. In addition, newly according dates colony follows: MOC 10-16, LOC 17-23 VOC 24-30. Flow cytometry analyses revealed expression patterns surface antigens relating hematopoietic/endothelial lineages varied among subpopulations, e.g., EOC: CD31 + CD34 CD14 CD105 low LOC: - high . Using in vitro assays, we found LOCs had proliferation tube formation potentials along eNOS. Then, each subpopulation was intravenously injected into immunocompromised mice 1, 3, 5 7 after unilateral hindlimb ischemia surgery (each 1×10 per time). Four weeks surgery, LOC-injected group showed significantly enhanced flow recovery (blood ratios ischemic/non ischemic leg: 1.02±0.02 [LOC group] versus 0.60±0.16 0.85±0.14 [other groups], P <0.05) augmented capillary collateral leg. To evaluate how much percentage (ECs) generated capillaries derived EPCs, GFP-labeled ischemia. The proportions human EPC-derived ECs a total muscle 14.3±2.8% 4.0±1.8% 9.2±2.3% other groups ( <0.05). Conclusions: Late ex vivo are superior