A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks

• DOI: 10.1161/circresaha.107.159475
• Publication Date: 2007-10-25T23:46:44Z
• Authors: Dmitry O. Traktuev; Stephanie Merfeld...; Jingling Li; Mikhail Kolonin; Wadih Arap; Renata Pasqualini; Brian H. Johnstone; Keith L. March
• Keywords: Matrigel; 3T3-L1; Stromal vascular fraction

It has been shown that stromal–vascular fraction isolated from adipose tissues contains an abundance of CD34 + cells. Histological analysis tissue revealed cells are widely distributed among adipocytes and predominantly associated with vascular structures. The majority freshly were CD31 − /CD144 could be separated a distinct population /CD31 (endothelial) by differential attachment on uncoated plastic. localization within suggested the nonendothelial these occupied pericytic position. Analysis surface intracellular markers adipose-derived stromal (ASCs) showed >90% coexpress mesenchymal (CD10, CD13, CD90), (chondroitin sulfate proteoglycan, CD140a, CD140b), smooth muscle (α-actin, caldesmon, calponin) markers. ASCs demonstrated polygonal self-assembly Matrigel, as did human microvascular endothelial Coculture Matrigel led to cooperative network assembly, enhanced stability networks preferential abluminal side cords. Bidirectional paracrine interaction between was supported identification angiogenic factors (vascular growth factor, hepatocyte basic fibroblast factor), inflammatory (interleukin-6 -8 monocyte chemoattractant protein-1 -2), mobilization (macrophage colony-stimulating factor granulocyte/macrophage factor) in media conditioned ASCs, well robust mitogenic response epidermal platelet-derived factor-BB, produced These results demonstrate for first time adherent resident pericytes play role stabilization mutual structural functional