Dependence of Cardiac Transverse Tubules on the BAR Domain Protein Amphiphysin II (BIN-1)

• DOI: 10.1161/circresaha.116.303448
• Publication Date: 2014-10-21T06:45:51Z
• Authors: Jessica L. Caldwell; Charlotte E.R. Smith; Rebecca F. Taylor; Ashraf Kitmitto; David A. Eisner; Katharine M. Dibb; Andrew W. Trafford
• Keywords: Amphiphysin; Distal convoluted tubule; Tubule

Transverse tubules (t-tubules) regulate cardiac excitation-contraction coupling and exhibit interchamber interspecies differences in expression. In disease, t-tubule loss occurs affects the systolic calcium transient. However, mechanisms controlling maintenance whether these factors differ between species, chambers, a disease setting remain unclear.To determine role of Bin/Amphiphysin/Rvs domain protein amphiphysin II (AmpII) regulating transient.T-tubule density was assessed by di-4-ANEPPS, FM4-64 or WGA staining using confocal microscopy. rat, ferret, sheep hearts AmpII levels were lower atrium than ventricle. Heart failure (HF) induced right ventricular tachypacing ferrets ascending aortic coarctation. both HF models, decreased ventricles. sheep, atrial t-tubules also lost decreased. Conversely, junctophilin 2 did not show rat ferret nor they change ferret. addition, cells where absent, had sarcomeric intracellular distribution. Small interfering RNA-induced knockdown reduced density, transient amplitude, synchrony transient.AmpII is intricately involved maintenance. Reducing decreases reduces increases heterogeneity