Noninvasive Assessment of Myocardial Inflammation by Cardiovascular Magnetic Resonance in a Rat Model of Experimental Autoimmune Myocarditis

• DOI: 10.1161/circulationaha.111.075283
• Publication Date: 2012-05-02T02:43:46Z
• Authors: Hyeyoung Moon; Hyo Eun Park; Jongeun Kang; Hyunseung Lee; Chaejoon Cheong; Yong Taik Lim; Sang-Hyun Ihm; Ki-Bae Seung; Farouc A. Jaffer; Jagat Narula; Kiyuk Chang; Kwan Soo Hong
• Keywords: Cardiac magnetic resonance

Background— Limited availability of noninvasive and biologically precise diagnostic tools poses a challenge for the evaluation management patients with myocarditis. Methods Results— The feasibility cardiovascular magnetic resonance (CMR) imaging magneto-fluorescent nanoparticles (MNPs) detection myocarditis its effectiveness in discriminating inflammation grades were assessed experimental autoimmune (EAM) (n=65) control (n=10) rats. After undergoing CMR, rats administered MNPs, followed by second CMR 24 hours later. Head-to-head comparison MNP-CMR T 2 -weighted, early late gadolinium enhancement was performed additional EAM (n=5) Contrast-to-noise ratios measured compared between groups. Flow cytometry microscopy demonstrated that infiltrating inflammatory cells engulfed resulting altered myocardial * effect. Changes contrast-to-noise ratio pre- post-MNP significantly greater (1.08±0.10 versus 0.48±0.20; P <0.001). In addition, measurement clearly detected extent ( <0.001) except mild inflammation. Compared conventional provided better image contrast (CNR change 8% 46%, detectability focal Notably, successfully tracked evolution same Conclusions— Magneto-fluorescent nanoparticle permitted effective visualization cellular infiltrates distinction preclinical model EAM. performs best at least moderate response.