Enhanced Survival of Transplanted Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes by the Combination of Cell Sheets With the Pedicled Omental Flap Technique in a Porcine Heart

• DOI: 10.1161/circulationaha.112.000366
• Publication Date: 2013-09-12T21:37:10Z
• Authors: Masashi Kawamura; Shigeru Miyagawa; Satsuki Fukushima; Atsuhiro Saito; Kenji Miki; Emiko Ito; Nagako Sougawa; Takuji Kawamura; Takashi Daimon; Tatsuya Shimizu; Teruo Okano; Koichi Toda; Yoshiki Sawa
• Keywords: Greater omentum

Background— Transplantation of cardiomyocytes that are derived from human induced pluripotent stem cell–derived (hiPS-CMs) shows promise in generating new functional myocardium situ, whereas the survival and functionality transplanted cells critical considering this therapeutic impact. Cell-sheet method has been used to transplant many cells; however, potential ischemia might limit cell survival. The omentum, which is known have rich vasculature, expected be a source blood supply. We hypothesized transplantation hiPS-CM sheets combined with an omentum flap may deliver large number hiPS-CMs enhanced Methods Results— Retrovirally established iPS were treated Wnt signaling molecules induce cardiomyogenic differentiation, followed by superparamagnetic iron oxide labeling. Cell created magnetically labeled using temperature-responsive dishes porcine hearts or without (n=8 each). Two months after transplantation, oxide–labeled hiPS-CMs, assessed MRI, was significantly greater mini-pigs than those it; histologically, vascular density area it. tissues contained abundant cardiac troponin T–positive surrounded vascular-rich structures. Conclusions— via increased angiogenesis, suggesting strategy useful clinical settings. combination promising technique for development tissue-engineered vivo.