Human Blood and Cardiac Stem Cells Synergize to Enhance Cardiac Repair When Cotransplanted Into Ischemic Myocardium

• DOI: 10.1161/circulationaha.112.000374
• Publication Date: 2013-09-12T21:37:10Z
• Authors: Nicholas Latham; Bin Ye; Robyn Jackson; Bu-Khanh Lam; Drew Kuraitis; Marc Ruel; Erik J. Suuronen; Duncan J. Stewart; Darryl R. Davis
• Keywords: Stem Cell Therapy; Cell therapy

Background— Blood-derived circulatory angiogenic cells (CACs) and resident cardiac stem (CSCs) have both been shown to improve function after myocardial infarction. The superiority of either cell type has long an area speculation with no definitive head-to-head trial. In this study, we compared the effect human CACs CSCs, alone or in combination, on immunodeficient mouse model Methods Results— CSCs were cultured from left atrial appendages blood samples obtained patients undergoing clinically indicated heart surgery. expressed a broader cytokine profile than 3 cytokines common. Coculture further enhanced production stromal cell–derived factor-1α vascular endothelial growth factor ( P ≤0.05). Conditioned media promoted equivalent networks CAC recruitment superior effects using cocultured conditioned media. Intramyocardial injection improved reduced scar burdens when injected 1 week infarction ≤0.05 versus negative controls). Cotransplantation together greater extent therapy CSC alone). Conclusions— provide unique paracrine repertoires angiogenesis, migration, repair. Combination types synergistically improves postinfarct alone. This synergy is likely mediated by complimentary signatures that promote revascularization new myocardium.