Oral Formulation of Angiotensin-(1–7) Improves Lipid Metabolism and Prevents High-Fat Diet–Induced Hepatic Steatosis and Inflammation in Mice

• DOI: 10.1161/hypertensionaha.111.00919
• Publication Date: 2013-06-11T05:18:01Z
• Authors: John David Felten...; João Marcus Olive...; Alanna Paraíso; Lucas Oliveira Ba...; Aristides Batista...; Ruben D.M. Sinist...; Frederico B. Sousa; André Luiz Sena G...; Alfredo Mauricio ...; Maria José Campag...; Mahboob Qureshi; Robson Augusto So...; Sérgio Henrique S...
• Keywords: Steatosis; Angiotensin 1

Angiotensin (Ang)-(1-7) has been described as an important tool on treating and preventing metabolic disorders. In this study, we aimed to evaluate the effect of oral formulation Ang-(1-7) included in hydroxypropylβ-cyclodextrin (HPβCD/Ang-[1-7]) hepatic function, steatosis, liver inflammatory markers expression mice treated with a high-fat diet. Male FVB/N were divided into 4 groups fed for 60 days, each group receiving 1 following diets: standard diet+HPβCD, diet+Ang-(1-7)/HPβCD, or diet+Ang-[1-7]/HPβCD. Body weight, food intake, blood parameters, such total cholesterol, triglyceride, alaninetransaminases, aspartate transaminases, evaluated. Immunohistochemical analyses performed tumor necrosis factor-α interleukin-6. Expression angiotensin converting enzyme, angiotensin-converting enzyme-2, interleukin-1β, factor-α, interleukin-6, transforming growth factor-β, acetyl-CoA carboxylase, carbohydrate-responsive element-binding protein, peroxisome proliferator-activated receptor-γ, sterol regulatory proteins-1c was evaluated by quantitative real-time polymerase chain reaction. The major findings our study reduced fat mass decreased plasma alaninetransaminase enzyme levels Ang-(1-7)-treated compared control groups. These results accompanied significant reduction interleukin-6 mRNA liver. Analyses adipogenesis-related genes reaction showed that significantly suppressed. conclusion, observed treatment improved metabolism proinflammatory profile deposition mice.