Abstract We112: Volumetric imaging to recapitulate cardiac injury and arrhythmias

• DOI: 10.1161/res.135.suppl_1.we112
• Publication Date: 2024-10-09T14:03:28Z
• Authors: Yichen Ding; Alireza Saberigar...; Xinyuan Zhang; Milad Almasian; Jie Yuan

Advanced understanding of cardiac structure and function is crucial for uncovering the underlying mechanism injury arrhythmias. To explore cues to structural functional abnormalities in extensively established animal models, we have developed optical imaging computational methods tailored investigate intact hearts zebrafish rodents at cellular resolution. Our light-field microscopy allows us capture instantaneous dynamics such as calcium transients, irregular contraction, blood flow 200 volumes per second larvae 1 . improve spatial resolution enable study rodent customized two light-sheet systems. One system empowered by retrospective synchronization machine learning contractile focal myocardial mechanics from end-systole end-diastole 2 , while other integrates tissue clearing axially scanning approaches exploration ventricular trabeculation compaction 3 These aim overcome trade-off resolution, speed, field view across atria ventricles with minimal photo-damage maximal penetration depth, enabling long-term investigation development regenerative processes. further interpret large volume datasets, a successive subspace virtual reality-based platform interactive image analysis 4 Collectively, our multi-scale strategy has opened up new landscapes exploring micro-structure function, both health disease. References 1. Saberigarakani A, et al. Light-field arrhythmias larvae. In: Three-Dimensional Multidimensional Microscopy: Image Acquisition Processing XXXI. 2024. p. 48–55. 2. Zhang X, 4D Light-sheet contractility APL Bioeng 2023;7:26112. 3. Almasian M, 3D isotropic reveal neonatal mouse hearts. PC128480F. 4. Yuan J, Extended reality biomedicine. Nat Rev Methods Prim 2023;3:14.