Abstract TMP71: Role Of Histone Deacetylases in Stroke-induced Oligodendrogenesis

• DOI: 10.1161/str.44.suppl_1.atmp71
• Publication Date: 2021-07-03T12:02:14Z
• Authors: Haifa Kassis; Michael Chopp; Xian Shuang Liu; Cynthia Roberts; Zheng Gang Zhang
• Keywords: HDAC1; HDAC4; Histone deacetylase 2; Stroke; Histone deacetylase 5

Background: Histone deacetylases (HDACs) are a superfamily of isoforms that deacetylate lysine residues in histones. HDAC activity is essential for oligodendrocyte (OLG) differentiation. The present study measured spatial profiles individual HDACs white matter during brain repair after stroke. Methods and Results: Adult male Wistar rats (n=8) were subjected to permanent right MCA occlusion sacrificed 8 days later. Immunohistochemistry was performed on coronal sections with antibodies against HDACs, NG2 (a marker progenitor cells, OPCs), APC OLGs). numbers immunoreactive cells counted peri-infarct contralateral corpus callosum. Double immunofluorescent staining revealed stroke significantly (p<0.05) increased the number HDAC1 (HDAC1+/NG2+ 166±7 vs.105±6 contralateral), HDAC2 (HDAC2+/NG2+ 130±4 vs. 98±2), HDAC4 (HDAC4+/NG2+ 74±5 56±4) positive OPCs, but did not change HDAC5 OPCs. However, only OLGs (HDAC2+/APC+ 466 ±15 420±10), whereas decreased (HDAC1+/APC 438±24 533±15, p<0.05). affects acetylation (Ac) status histone proteins (HH) within chromatin. We found Ac-HH3+ OPCs (49±2 64±2) (311±16 356±13), which consistent elevation both cell types. Conclusion: Our data indicate induces different expression class I (1 2) II (4 5) OLGs, suggesting these could play distinct roles stroke-induced oligodendrogenesis.