Abstract TP6: Thymosin alpha 1 promotes neuron survival by enhancing mitophagy after AIS

• DOI: 10.1161/str.56.suppl_1.tp6
• Publication Date: 2025-01-30T10:11:28Z
• Authors: xinmei Kang; Shisi Wang; Wei Cai; Zhengqi Lu
• Keywords: Alpha (finance); Stroke

Background: Thymosin alpha 1 (Tα1) had been used in kinds of inflammatory and immunodeficiency diseases as an immunomodulator. According to our clinic observation, patients with acute ischemic stroke (AIS) treated Tα1 prevent/cure post-stroke infection display favorable outcome. Whether exerts direct neuronal protection besides immunomodulation remains elusive. Aim: To explore the effect on after AIS unveil underlying mechanisms. Method: In vivo : 8-10 weeks old male Wild type (WT) mice were subjected 60 minutes middle cerebral artery occlusion (tMCAO) administrated Tα-1 by intraperitoneal injection. Ex Primary mouse neuron is min oxygen glucose deprivation (OGD), followed treatment. Results: Compared untreated group, treatment displayed decreased infarct volume, limited loss, attenuated white matter injury，restricted neuroinflammation improved neurological functions. Treatment antibiotics or glucocorticoid failed substitute therapeutic effects Tα1, indicating extra anti-infection immunomodulation. As for primary neuron, showed fewer death higher cell viability upon OGD stimulation. Both ex experiments revealed that directly inhibited ischemia-induced death. Mechanistically, enhanced mitophagy down-regulating Drp1, thus promoted mitochondrial renewal supported viability. Conclusions: Our results identified possessed prognosis AIS. Given multi-functions including anti-infection, protection, we propose a promising therapy against