Protecting Against Cerebrovascular Injury

• DOI: 10.1161/strokeaha.108.514927
• Publication Date: 2008-07-18T02:05:24Z
• Authors: Guang Jin; Ken Arai; Yoshihiro Murata; Sophia Wang; Monique F. Stins; Eng H. Lo; Klaus van Leyen
• Keywords: Stroke

Background and Purpose— The concept of the neurovascular unit suggests that effects on brain vasculature must be considered if neuroprotection is to achieved in stroke. We previously reported 12/15-lipoxygenase (12/15-LOX) upregulated peri-infarct area after middle cerebral artery occlusion mice, 12/15-LOX contributes damage ischemia–reperfusion. current study was designed investigate involvement vascular injury ischemic brain. Methods— In cell culture, a human microvascular endothelial line subjected either hypoxia or H 2 O -induced oxidative stress with without lipoxygenase inhibitors. For vivo studies, mice were 90 minutes occlusion, gene knockout treatment inhibitors compared. Expression claudin-5 as well extravasation immunoglobulin G detected by immunohistochemistry. Edema measured water content hemispheres according wet–dry weight method. Results— Brain cells protected against inhibitor baicalein. After focal ischemia, increased neurons cells. tight junction protein underwent extensive degradation area, which partially prevented Leakage into parenchyma significantly reduced wild-type treated Likewise, edema ameliorated. Conclusion— may contribute not just causing neuronal death, but also detrimental microvasculature. thus effective both neuroprotectants vasculoprotectants.