Inhibition of cell growth and induction of apoptosis by acacetin in FaDu human pharyngeal carcinoma cells

• DOI: 10.11620/ijob.2020.45.3.107
• Publication Date: 2020-10-21T05:36:46Z
• Authors: Kyeong-Rok Kang; Jae-Sung Kim; Tae-Hyeon Kim; Jeong-Yeon Seo; Jong-Hyun Park; Jin Woong Lim; Sun-Kyoung Yu; Heung-Joong Kim; Sang Hun Shin; Bo-Ram Park; Chun Sung Kim; Do Kyung Kim
• Keywords: Acacetin

Acacetin, which is present in damiana (Turnera diffusa ) and black locust (Robinia pseudoacacia ), has several pharmacologic activities such as antioxidant, anti-inflammatory, anti-proliferative effects on cancer cells. However, the effect of acacetin head neck cancers not been clearly established. This study aimed to examine cell growth apoptosis induction FaDu human pharyngeal carcinoma These were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, Live/Dead 4′,6-diamidino-2-phenylindole dihydrochloride staining, caspase-3 caspase-7 activation immunoblotting Acacetin induced death a dose-dependent manner, with an estimated IC50 value 41.9 µM, without affecting viability L-929 mouse fibroblasts normal treatment resulted nuclear condensation It promoted proteolytic cleavage procaspase-3, -7, -8, -9 increasing amounts cleaved caspase isoforms Acacetin-induced cells was mediated expression Fas caspase-8, caspase-3, poly (ADP-ribose) polymerase. Immunoblotting showed downregulation anti-apoptotic mitochondrial proteins Bcl-2 Bcl-xL, but upregulation mitochondria-dependent pro-apoptotic Bax Badin after treatment. findings indicate that inhibits proliferation induces apoptotic via both receptor-mediated extrinsic pathway mitochondria-mediated intrinsic pathway.