Oxidative Stress and Respiratory Muscle Dysfunction in Severe Chronic Obstructive Pulmonary Disease

• DOI: 10.1164/rccm.200407-887oc
• Publication Date: 2005-02-26T01:34:06Z
• Authors: Esther Barreiro; Beatriz de la Puente; Joan Minguella; Josep M. Corominas; Sergi Serrano; Sabah N. A. Hussain; Joaquim Gea
• Keywords: Nitrotyrosine

Oxidative stress is involved in the skeletal muscle dysfunction observed patients with severe chronic obstructive pulmonary disease (COPD). We hypothesized that diaphragms of such might generate greater levels oxidants than those neutralized by antioxidants.To assess both oxidative and nitrosative different antioxidants patients, to analyze potential relationships lung respiratory dysfunctions.We conducted a case-control study which reactive carbonyl groups, hydroxynonenal-protein adducts, antioxidant enzyme levels, nitric oxide synthases, 3-nitrotyrosine formation were detected using immunoblotting immunhistochemistry diaphragm specimens (thoracotomy) obtained from six COPD, moderate seven control subjects.Diaphragms COPD showed higher protein groups adducts subjects. When only considering negative correlations found between airway obstruction, strength. Although diaphragmatic neuronal synthase did not differ among three no inducible was any muscle, endothelial lower Muscle nitrotyrosine similar subjects.This shows rather likely be COPD.