The CXCL10/CXCR3 Axis Mediates Human Lung Mast Cell Migration to Asthmatic Airway Smooth Muscle

• DOI: 10.1164/rccm.200409-1220oc
• Publication Date: 2005-02-03T01:12:36Z
• Authors: Christopher E. Br...; Alaina J. Ammit; Davinder Kaur; Judith L. Black; Andrew J. Wardlaw; J. Margaret Hughes; Peter Bradding
• Keywords: CXCR3; CXC chemokine receptors; Ex vivo

Mast cell microlocalization within the airway smooth muscle bundle is an important determinant of asthmatic phenotype. We hypothesized that mast cells migrate toward in response to muscle-derived chemokines. In this study, we investigated (1) chemokine receptor expression by bronchial biopsies from subjects with asthma using immunohistology, (2) concentration chemokines supernatants stimulated ex vivo and without measured enzyme-linked immunosorbent assay, (3) migration these chemotaxis assays. found CXCR3 was most abundantly expressed on human lung 100% compared 47% submucosa. Human induced cultures predominantly through activation CXCR3. Most importantly, CXCL10 preferentially those healthy control subjects. These results suggest inhibition CXCL10/CXCR3 axis offers a novel target for treatment asthma.