Intermittent Hypoxia Causes Insulin Resistance in Lean Mice Independent of Autonomic Activity

• DOI: 10.1164/rccm.200610-1527oc
• Publication Date: 2007-02-03T06:47:56Z
• Authors: Nao Iiyori; Laura C. Alonso; Jianguo Li; Mark H. Sanders; Adolfo Garcia-Ocana; Robert M. O'Doherty; Vsevolod Y. Polotsky; Christopher P. O'...
• Keywords: Intermittent hypoxia; Hypoxia

Rationale and Objectives: Although many clinical physiology epidemiology studies show an association between obstructive sleep apnea (OSA) markers of insulin resistance, no causal pathway has been established. The purpose the current study was to determine if intermittent hypoxia (IH) stimulus that characterizes OSA causes resistance in absence obesity. Furthermore, we assessed impact IH on specific metabolic function liver muscle. Finally, examined potential mechanistic role autonomic nervous system (ANS) mediating response IH.Methods Results: Hyperinsulinemic euglycemic clamps were conducted whole-body sensitivity, hepatic glucose output, muscle-specific utilization conscious, chronically instrumented adult male C57BL/6J mice exposed (1) (achieving a nadir FiO2 = 5–6% at 60 cycles/h for 9 h), (2) air as control, (3) with ANS blockade (hexamethonium), or (4) IA blockade. decreased sensitivity compared (38.8 ± 2.7 vs. 49.4 1.5 mg/kg/min, p < 0.005) reduced oxidative muscle fibers, but did not cause change output. reduction during restored by blockade.Conclusion: We conclude can acute otherwise lean, healthy animals, is associated it occurs independently activation ANS.