Bacteria Challenge in Smoke-exposed Mice Exacerbates Inflammation and Skews the Inflammatory Profile

• DOI: 10.1164/rccm.200808-1306oc
• Publication Date: 2009-01-30T02:10:45Z
• Authors: Gordon J. Gaschler; Marko Skrtic; Caleb C. J. Zavitz; Maria Lindahl; Per-Ola Onnervik; Timothy F. Murphy; Sanjay Sethi; Martin R. Stämpfli
• Keywords: Ex vivo; Pathogenesis; Mediator

Rationale: The pathogenesis of chronic obstructive pulmonary disease is associated with acute episodes bacterial exacerbations. most commonly isolated bacteria during exacerbation nontypeable Haemophilus influenzae (NTHI).Objectives: In this study, we investigated the in vivo consequences cigarette smoke exposure on inflammatory response to an NTHI challenge.Methods: C57BL/6 and BALB/c mice were exposed for 8 weeks subsequently challenged intranasally NTHI.Measurements Main Results: We observed increased inflammation lung damage smoke–exposed NTHI-challenged as compared control mice. Furthermore, although challenge was marked by increases tumor necrosis factor-α, IL-6, MIP-2, KC/GROα, led a prominent up-regulation different subset mediators, notably MCP-1, -3, -5, IP-10, MIP-1γ. This skewed mediator expression also after ex stimulation alveolar macrophages, signifying their importance altered response. Importantly, corticosteroids attenuated both mice; however, significantly burden.Conclusions: Collectively, these data suggest that exacerbates via expression.