Comparison of the Respiratory Microbiome in Healthy Nonsmokers and Smokers

• DOI: 10.1164/rccm.201210-1913oc
• Publication Date: 2013-03-15T10:11:06Z
• Authors: Alison Morris; James M. Beck; Patrick D. Schloss; Thomas B. Campbell; Kristina Crothers; Jeffrey L. Curtis; Sonia C. Flores; Andrew P. Fontenot; Elodie Ghedin; Laurence Huang; Kathleen Jablonski; Eric Kleerup; Susan V. Lynch; Erica Sodergren; Homer Twigg; Vincent B. Young; Christine M. Bassis; Arvind Venkataraman; Thomas M. Schmidt; George M. Weinstock
• Keywords: Lung HIV Microbiome Project; Male; 0301 basic medicine; bronchoscopy; Respiratory System; microbiome; Clinical sciences; Medical and Health Sciences; Cohort Studies; Reference Values; Cardiovascular Medicine and Haematology; 80 and over; 2.2 Factors relating to the physical environment; Prospective Studies; Aetiology; Lung; Aged, 80 and over; Smoking; Middle Aged; 3. Good health; Infectious Diseases; Respiratory; Female; Infection; Sequence Analysis; Bronchoalveolar Lavage Fluid; Adult; Adolescent; Clinical Sciences; 610; Cardiovascular medicine and haematology; metagenome; lung; Young Adult; 03 medical and health sciences; Clinical Research; Tobacco; Genetics; Humans; Aged; Mouth; Tobacco Smoke and Health; Biomedical and Clinical Sciences; Human Genome; DNA; Sequence Analysis, DNA; Metagenome; Microbiome

Rationale: Results from 16S rDNA-encoding gene sequence–based, culture-independent techniques have led to conflicting conclusions about the composition of lower respiratory tract microbiome.Objectives: To compare microbiome upper and in healthy HIV-uninfected nonsmokers smokers a multicenter cohort.Methods: Participants were without significant comorbidities. Oral washes bronchoscopic alveolar lavages collected standardized manner. Sequence analysis bacterial rRNA-encoding genes was performed, neutral model community ecology used identify bacteria that most plausible members lung microbiome.Measurements Main Results: Sixty-four participants enrolled. Most identified also mouth, but specific such as Enterobacteriaceae, Haemophilus, Methylobacterium, Ralstonia species disproportionally represented lungs compared with values predicted by model. Tropheryma lung, not mouth. Mouth communities differed between Porphyromonas, Neisseria, Gemella, populations did not.Conclusions: This study is largest examine individuals first use Specific appear significantly higher abundance than would be expected if they originated demonstrating does derive entirely The mouth differs smokers, altered smoking.