Genetic Stability of Cassava Plants Regenerated Through Organogenesis Using Microsatellite Markers

• DOI: 10.11648/j.jps.20170501.13
• Publication Date: 2017-01-23
• Authors: Gilbert Osena; Nelson O. Amugune; Evans N. Nyaboga
• Keywords: Somaclonal variation; Subculture (biology); Germ plasm; Organogenesis

Tissue culture technology of cassava (Manihot esculenta Crantz) is a viable alternative to currently adopted techniques for mass propagation, germplasm conservation and genetic improvement. However, somaclonal variation common phenomenon in tissue which makes it mandatory monitor the stability plants. Therefore, objective this study was evaluate plants regenerated from axillary bud explants through direct organogenesis using simple sequence repeat (SSR) markers. High shoot regeneration (81.2 – 90.0%) occurred MS medium supplemented with 10 mg/L 6-bnzylaminopurine (BAP) multiple shoots (2 4 per explant) were formed all three cultivars (TME14, TMS60444 Kibandameno) tested. frequency rooting (100%) obtained after transferring plantlets basic (CBM) rooted successfully hardened acclimatized glasshouse 100% survival rate. Three-month old exhibited normal morphological characters comparing mother plant. A total SSR markers used validate homogeneity amongst five randomly selected along donor DNA fingerprints displayed monomorphic bands similar plant, indicating among The effect subculture on bud-derived regenerants micropropagated also assessed All profiles comparable 1st 5th subculture, confirming clones At 6th similarity indicators between progenies ranged 0.95 1.0 such indicated very low polymorphism. dendrograms generated Unweighted Pair Group Method arithmetic mean (UPGMA) analysis revealed 96% This polymorphism ratio plants, micro-propagated indicates little variations, high demonstrates reliability propagation system cassava. These results suggest that buds safest method true-to-type can be clonal transformation