Asbestos fibers and interferon-gamma up-regulate nitric oxide production in rat alveolar macrophages.

• DOI: 10.1165/ajrcmb.11.6.7524571
• Publication Date: 2013-04-04T02:29:35Z
• Authors: G Thomas; T Ando; K Verma; E Kagan
• Keywords: Chrysotile; Hydroxyl radical; Griess test; Alveolar macrophage

The present study was undertaken to determine whether asbestos exposure induces the formation of nitric oxide (NO.) radical by rat alveolar macrophages (AM). For this purpose, AM from Sprague-Dawley rats were cultured for 48 h in presence or absence either chrysotile (serpentine) crocidolite (amphibole) fibers. effects fibers compared with those nonfibrogenic carbonyl iron particles. Nitrite (NO2-), stable oxidation product NO. macrophage conditioned medium, assayed Griess reaction. Production NO2- significantly increased both (P < 0.01) and 0.05) (10 micrograms/ml). Since interferon-gamma (IFN-gamma) is known induce synthase within macrophages, since elevated levels intrapulmonary IFN-gamma have been noted workers, combined also studied context formation. Addition (250 500 IU/ml) synergistically enhanced induced crocidolite. Notably, had no significant effect on production AM. attenuated inhibitor, NG-monomethyl-L-arginine (0.5 1 mg/ml). By contrast, superoxide dismutase (150 U/ml) asbestos-induced 0.001). anion can interact generate toxic hydroxyl radical, protect against injury, induction may represent a novel form asbestos-related injury.