Mucous and serous secretions of human bronchial epithelial cells in secondary culture.

• DOI: 10.1165/ajrcmb.12.2.7865212
• Publication Date: 2013-04-04T02:28:34Z
• Authors: N Emery; G A Place; S Dodd; M Lhermitte; G David; G Lamblin; J M Perini; A M Page; R L Hall; P Roussel
• Keywords: Glycoconjugate

Human bronchial surface epithelial cells were maintained in secondary culture on a collagen gel substrate defined, serum-free medium. These conditions have previously been reported to promote mucous cell differentiation. After 3 wk culture, approximately 40% of the stained by an antibody directed against human respiratory mucin. Analysis media from cultured presence radioactive precursors [3H]glucosamine and [35S]sulfate revealed that secreted high molecular weight glycoproteins with properties typical mucins. In addition, hyaluronic acid proteoglycans containing chondroitin sulfate and/or heparan glycosaminoglycans identified conditioned media. Finally, Western blot analyses showed lysozyme proteinase inhibitor, proteins are generally considered be markers for submucosal gland serous cells. results show epithelium range glycoconjugates secretory products both